Erratum: Corrigendum: Optimized orthogonal translation of unnatural amino acids enables spontaneous protein double-labelling and FRET
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چکیده
منابع مشابه
Molecular Evolution Directs Protein Translation Using Unnatural Amino Acids.
Unnatural amino acids have in recent years established their importance in a wide range of fields, from pharmaceuticals to polymer science. Unnatural amino acids can increase the number of chemical groups within proteins and thus expand or enhance biological function. Our ability to utilize these important building blocks, however, has been limited by the inherent difficulty in incorporating th...
متن کاملUnnatural Protein Engineering: Producing Proteins with Unnatural Amino Acids
Less than a decade ago, the ability to generate proteins with unnatural modifications was a Herculean task available only to specialty labs. Recent advances make it possible to generate reasonable quantities of protein with unnatural amino acids both in vitro and in vivo . The combination of solid-phase peptide synthesis and enzymatic or chemoselective ligation now permits construction of entir...
متن کاملProbing protein stability with unnatural amino acids.
Unnatural amino acid mutagenesis, in combination with molecular modeling and simulation techniques, was used to probe the effect of side chain structure on protein stability. Specific replacements at position 133 in T4 lysozyme included (i) leucine (wt), norvaline, ethylglycine, and alanine to measure the cost of stepwise removal of methyl groups from the hydrophobic core, (ii) norvaline and O-...
متن کاملSynthesis and Protein Incorporation of Azido-Modified Unnatural Amino Acids.
Two new azidophenylalanine residues (3 and 4) have been synthesized and, in combination with 4-azido-L-phenylalanine (1) and 4-azidomethyl-L-phenylalanine (2), form a series of unnatural amino acids (UAAs) containing the azide vibrational reporter at varying distances from the aromatic ring of phenylalanine. These UAAs were designed to probe protein hydration with high spatial resolution by uti...
متن کاملSite-specific protein immobilization using unnatural amino acids.
Protein immobilization confers the advantages of biological systems to a more chemical setting and has applications in catalysis, sensors, and materials development. While numerous immobilization techniques exist, it is optimal to develop a well-defined and chemically stable methodology to allow for full protein function. This paper describes the utilization of unnatural amino acid technologies...
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ژورنال
عنوان ژورنال: Nature Chemistry
سال: 2014
ISSN: 1755-4330,1755-4349
DOI: 10.1038/nchem.2175